A versatile workflow to integrate RNA-seq genomic and transcriptomic data into mechanistic models of signaling pathways.

TitleA versatile workflow to integrate RNA-seq genomic and transcriptomic data into mechanistic models of signaling pathways.
Publication TypeJournal Article
Year of Publication2021
AuthorsGarrido-Rodriguez, M, López-López, D, Ortuno, FM, Peña-Chilet, M, Muñoz, E, Calzado, MA, Dopazo, J
JournalPLoS Comput Biol
Volume17
Issue2
Paginatione1008748
Date Published2021 Feb 11
ISSN1553-7358
Abstract

MIGNON is a workflow for the analysis of RNA-Seq experiments, which not only efficiently manages the estimation of gene expression levels from raw sequencing reads, but also calls genomic variants present in the transcripts analyzed. Moreover, this is the first workflow that provides a framework for the integration of transcriptomic and genomic data based on a mechanistic model of signaling pathway activities that allows a detailed biological interpretation of the results, including a comprehensive functional profiling of cell activity. MIGNON covers the whole process, from reads to signaling circuit activity estimations, using state-of-the-art tools, it is easy to use and it is deployable in different computational environments, allowing an optimized use of the resources available.

DOI10.1371/journal.pcbi.1008748
Alternate JournalPLoS Comput Biol
PubMed ID33571195