SNP and haplotype mapping for genetic analysis in the rat

TitleSNP and haplotype mapping for genetic analysis in the rat
Publication TypeJournal Article
Year of Publication2008
AuthorsSaar, K, Beck, A, Bihoreau, MT, Birney, E, Brocklebank, D, Chen, Y, Cuppen, E, Demonchy, S, Dopazo, J, Flicek, P, Foglio, M, Fujiyama, A, Gut, IG, Gauguier, D, Guigo, R, Guryev, V, Heinig, M, Hummel, O, Jahn, N, Klages, S, Kren, V, Kube, M, Kuhl, H, Kuramoto, T, Kuroki, Y, Lechner, D, Lee, YA, Lopez-Bigas, N, Lathrop, GM, Mashimo, T, Medina, I, Mott, R, Patone, G, Perrier-Cornet, JA, Platzer, M, Pravenec, M, Reinhardt, R, Sakaki, Y, Schilhabel, M, Schulz, H, Serikawa, T, Shikhagaie, M, Tatsumoto, S, Taudien, S, Toyoda, A, Voigt, B, Zelenika, D, Zimdahl, H, Hubner, N
JournalNat Genet
Volume40
Pagination560-6
KeywordsAnimals Chromosome Mapping *Databases; Genetic; Genetic Genome *Haplotypes Linkage Disequilibrium Phylogeny *Polymorphism; Inbred Strains/*genetics Recombination; Single Nucleotide *Quantitative Trait Loci Rats/*genetics Rats
Abstract

The laboratory rat is one of the most extensively studied model organisms. Inbred laboratory rat strains originated from limited Rattus norvegicus founder populations, and the inherited genetic variation provides an excellent resource for the correlation of genotype to phenotype. Here, we report a survey of genetic variation based on almost 3 million newly identified SNPs. We obtained accurate and complete genotypes for a subset of 20,238 SNPs across 167 distinct inbred rat strains, two rat recombinant inbred panels and an F2 intercross. Using 81% of these SNPs, we constructed high-density genetic maps, creating a large dataset of fully characterized SNPs for disease gene mapping. Our data characterize the population structure and illustrate the degree of linkage disequilibrium. We provide a detailed SNP map and demonstrate its utility for mapping of quantitative trait loci. This community resource is openly available and augments the genetic tools for this workhorse of physiological studies.

Notes

STAR Consortium Saar, Kathrin Beck, Alfred Bihoreau, Marie-Therese Birney, Ewan Brocklebank, Denise Chen, Yuan Cuppen, Edwin Demonchy, Stephanie Dopazo, Joaquin Flicek, Paul Foglio, Mario Fujiyama, Asao Gut, Ivo G Gauguier, Dominique Guigo, Roderic Guryev, Victor Heinig, Matthias Hummel, Oliver Jahn, Niels Klages, Sven Kren, Vladimir Kube, Michael Kuhl, Heiner Kuramoto, Takashi Kuroki, Yoko Lechner, Doris Lee, Young-Ae Lopez-Bigas, Nuria Lathrop, G Mark Mashimo, Tomoji Medina, Ignacio Mott, Richard Patone, Giannino Perrier-Cornet, Jeanne-Antide Platzer, Matthias Pravenec, Michal Reinhardt, Richard Sakaki, Yoshiyuki Schilhabel, Markus Schulz, Herbert Serikawa, Tadao Shikhagaie, Medya Tatsumoto, Shouji Taudien, Stefan Toyoda, Atsushi Voigt, Birger Zelenika, Diana Zimdahl, Heike Hubner, Norbert 057733/Z/99/A/Wellcome Trust/United Kingdom 066780/Z/01/Z/Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov’t Technical Report United States Nature genetics Nat Genet. 2008 May;40(5):560-6.

URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18443594