TY - JOUR T1 - A Comprehensive DNA Methylation Profile of Epithelial-to-Mesenchymal Transition. JF - Cancer research Y1 - 2014 A1 - Carmona, F Javier A1 - Davalos, Veronica A1 - Vidal, Enrique A1 - Gomez, Antonio A1 - Heyn, Holger A1 - Hashimoto, Yutaka A1 - Vizoso, Miguel A1 - Martinez-Cardus, Anna A1 - Sayols, Sergi A1 - Ferreira, Humberto A1 - Sanchez-Mut, Jose A1 - Moran, Sebastian A1 - Margeli, Mireia A1 - Castella, Eva A1 - Berdasco, Maria A1 - Stefansson, Olafur Andri A1 - Eyfjord, Jorunn E A1 - Gonzalez-Suarez, Eva A1 - Dopazo, Joaquin A1 - Orozco, Modesto A1 - Gut, Ivo A1 - Esteller, Manel KW - Methyl-Seq KW - Methylomics KW - Next Generation Sequencing AB - Epithelial-to-mesenchymal transition (EMT) is a plastic process in which fully differentiated epithelial cells are converted into poorly differentiated, migratory and invasive mesenchymal cells and it has been related to the metastasis potential of tumors. This is a reversible process and cells can also eventually undergo mesenchymal-to-epithelial transition (MET). The existence of a dynamic EMT process suggests the involvement of epigenetic shifts in the phenotype. Herein, we obtained the DNA methylomes at single-base resolution of MDCK cells undergoing epithelial-to-mesenchymal transition (EMT) and translated the identified differentially methylated regions (DMRs) to human breast cancer cells undergoing a gain of migratory and invasive capabilities associated with the EMT phenotype. We noticed dynamic and reversible changes of DNA methylation, both on promoter sequences and gene-bodies in association with transcription regulation of EMT-related genes. Most importantly, the identified DNA methylation markers of EMT were present in primary mammary tumors in association with the epithelial or the mesenchymal phenotype of the studied breast cancer samples. VL - 74 UR - http://www.ncbi.nlm.nih.gov/pubmed/25106427 ER -