TY - JOUR T1 - Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate JF - Microb Drug Resist Y1 - 2001 A1 - Dopazo, J. A1 - Mendoza, A. A1 - Herrero, J. A1 - Caldara, F. A1 - Humbert, Y. A1 - Friedli, L. A1 - Guerrier, M. A1 - Grand-Schenk, E. A1 - Gandin, C. A1 - de Francesco, M. A1 - Polissi, A. A1 - Buell, G. A1 - Feger, G. A1 - Garcia, E. A1 - Peitsch, M. A1 - Garcia-Bustos, J. F. KW - Bacterial Molecular Sequence Data Pneumococcal Infections/*microbiology Prokaryotic Cells RNA KW - Bacterial/chemistry/genetics Genes KW - Bacterial/genetics *Genome KW - DNA KW - Transfer/metabolism Streptococcus pneumoniae/*genetics AB - The public availability of numerous microbial genomes is enabling the analysis of bacterial biology in great detail and with an unprecedented, organism-wide and taxon-wide, broad scope. Streptococcus pneumoniae is one of the most important bacterial pathogens throughout the world. We present here sequences and functional annotations for 2.1-Mbp of pneumococcal DNA, covering more than 90% of the total estimated size of the genome. The sequenced strain is a clinical isolate resistant to macrolides and tetracycline. It carries a type 19F capsular locus, but multilocus sequence typing for several conserved genetic loci suggests that the strain sequenced belongs to a pneumococcal lineage that most often expresses a serotype 15 capsular polysaccharide. A total of 2,046 putative open reading frames (ORFs) longer than 100 amino acids were identified (average of 1,009 bp per ORF), including all described two-component systems and aminoacyl tRNA synthetases. Comparisons to other complete, or nearly complete, bacterial genomes were made and are presented in a graphical form for all the predicted proteins. VL - 7 UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11442348 N1 - Dopazo, J Mendoza, A Herrero, J Caldara, F Humbert, Y Friedli, L Guerrier, M Grand-Schenk, E Gandin, C de Francesco, M Polissi, A Buell, G Feger, G Garcia, E Peitsch, M Garcia-Bustos, J F United States Microbial drug resistance (Larchmont, N.Y.) Microb Drug Resist. 2001 Summer;7(2):99-125. ER -