01868nas a2200145   4500008004100000245008600041210006900127260001300196300001000209490000700219520134300226100001701569700002501586856011101611       2011                        eng d00aStructure determination of genomic domains by satisfaction of spatial restraints.0 aStructure determination of genomic domains by satisfaction of sp  c2011 Jan  a25-350 v193 a<p>The three-dimensional (3D) architecture of a genome is non-random and known to facilitate the spatial colocalization of regulatory elements with the genes they regulate. Determining the 3D structure of a genome may therefore probe an essential step in characterizing how genes are regulated. Currently, there are several experimental and theoretical approaches that aim at determining the 3D structure of genomes and genomic domains; however, approaches integrating experiments and computation to identify the most likely 3D folding of a genome at medium to high resolutions have not been widely explored. Here, we review existing methodologies and propose that the integrative modeling platform ( http://www.integrativemodeling.org ), a computational package developed for structurally characterizing protein assemblies, could be used for integrating diverse experimental data towards the determination of the 3D architecture of genomic domains and entire genomes at unprecedented resolution. Our approach, through the visualization of looping interactions between distal regulatory elements, will allow for the characterization of global chromatin features and their relation to gene expression. We illustrate our work by outlining the recent determination of the 3D architecture of the &alpha;-globin domain in the human genome.</p>1 aBaù, Davide1 aMarti-Renom, Marc, A  uhttps://clinbioinfosspa.es/content/structure-determination-genomic-domains-satisfaction-spatial-restraints