<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Brumos, J.</style></author><author><style face="normal" font="default" size="100%">Colmenero-Flores, J. M.</style></author><author><style face="normal" font="default" size="100%">A. Conesa</style></author><author><style face="normal" font="default" size="100%">Izquierdo, P.</style></author><author><style face="normal" font="default" size="100%">Sanchez, G.</style></author><author><style face="normal" font="default" size="100%">Iglesias, D. J.</style></author><author><style face="normal" font="default" size="100%">Lopez-Climent, M. F.</style></author><author><style face="normal" font="default" size="100%">Gomez-Cadenas, A.</style></author><author><style face="normal" font="default" size="100%">Talon, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks</style></title><secondary-title><style face="normal" font="default" size="100%">Funct Integr Genomics</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=19190944</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Salinity tolerance in Citrus is strongly related to leaf chloride accumulation. Both chloride homeostasis and specific genetic responses to Cl(-) toxicity are issues scarcely investigated in plants. To discriminate the transcriptomic network related to Cl(-) toxicity and salinity tolerance, we have used two Cl(-) salt treatments (NaCl and KCl) to perform a comparative microarray approach on two Citrus genotypes, the salt-sensitive Carrizo citrange, a poor Cl(-) excluder, and the tolerant Cleopatra mandarin, an efficient Cl(-) excluder. The data indicated that Cl(-) toxicity, rather than Na(+) toxicity and/or the concomitant osmotic perturbation, is the primary factor involved in the molecular responses of citrus plant leaves to salinity. A number of uncharacterized membrane transporter genes, like NRT1-2, were differentially regulated in the tolerant and the sensitive genotypes, suggesting its potential implication in Cl(-) homeostasis. Analyses of enriched functional categories showed that the tolerant rootstock induced wider stress responses in gene expression while repressing central metabolic processes such as photosynthesis and carbon utilization. These features were in agreement with phenotypic changes in the patterns of photosynthesis, transpiration, and stomatal conductance and support the concept that regulation of transpiration and its associated metabolic adjustments configure an adaptive response to salinity that reduces Cl(-) accumulation in the tolerant genotype.&lt;/p&gt;</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;Journal article Functional &amp;amp; integrative genomics Funct Integr Genomics. 2009 Feb 4.&lt;/p&gt;</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Terol, J.</style></author><author><style face="normal" font="default" size="100%">A. Conesa</style></author><author><style face="normal" font="default" size="100%">Colmenero, J. M.</style></author><author><style face="normal" font="default" size="100%">Cercos, M.</style></author><author><style face="normal" font="default" size="100%">Tadeo, F.</style></author><author><style face="normal" font="default" size="100%">Agusti, J.</style></author><author><style face="normal" font="default" size="100%">Alos, E.</style></author><author><style face="normal" font="default" size="100%">Andres, F.</style></author><author><style face="normal" font="default" size="100%">Soler, G.</style></author><author><style face="normal" font="default" size="100%">Brumos, J.</style></author><author><style face="normal" font="default" size="100%">Iglesias, D. J.</style></author><author><style face="normal" font="default" size="100%">Gotz, S.</style></author><author><style face="normal" font="default" size="100%">Legaz, F.</style></author><author><style face="normal" font="default" size="100%">Argout, X.</style></author><author><style face="normal" font="default" size="100%">Courtois, B.</style></author><author><style face="normal" font="default" size="100%">Ollitrault, P.</style></author><author><style face="normal" font="default" size="100%">Dossat, C.</style></author><author><style face="normal" font="default" size="100%">Wincker, P.</style></author><author><style face="normal" font="default" size="100%">Morillon, R.</style></author><author><style face="normal" font="default" size="100%">Talon, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Analysis of 13000 unique Citrus clusters associated with fruit quality, production and salinity tolerance</style></title><secondary-title><style face="normal" font="default" size="100%">BMC Genomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acclimatization/*genetics Amino Acid Motifs Citrus/*genetics Cluster Analysis Expressed Sequence Tags Fruit/genetics Gene Duplication *Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Gene Library Genes</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Genomics Molecular Sequence Data Multigene Family Phylogeny *Salts/adverse effects</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=17254327</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">31</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND: Improvement of Citrus, the most economically important fruit crop in the world, is extremely slow and inherently costly because of the long-term nature of tree breeding and an unusual combination of reproductive characteristics. Aside from disease resistance, major commercial traits in Citrus are improved fruit quality, higher yield and tolerance to environmental stresses, especially salinity. RESULTS: A normalized full length and 9 standard cDNA libraries were generated, representing particular treatments and tissues from selected varieties (Citrus clementina and C. sinensis) and rootstocks (C. reshni, and C. sinenis x Poncirus trifoliata) differing in fruit quality, resistance to abscission, and tolerance to salinity. The goal of this work was to provide a large expressed sequence tag (EST) collection enriched with transcripts related to these well appreciated agronomical traits. Towards this end, more than 54000 ESTs derived from these libraries were analyzed and annotated. Assembly of 52626 useful sequences generated 15664 putative transcription units distributed in 7120 contigs, and 8544 singletons. BLAST annotation produced significant hits for more than 80% of the hypothetical transcription units and suggested that 647 of these might be Citrus specific unigenes. The unigene set, composed of  13000 putative different transcripts, including more than 5000 novel Citrus genes, was assigned with putative functions based on similarity, GO annotations and protein domains CONCLUSION: Comparative genomics with Arabidopsis revealed the presence of putative conserved orthologs and single copy genes in Citrus and also the occurrence of both gene duplication events and increased number of genes for specific pathways. In addition, phylogenetic analysis performed on the ammonium transporter family and glycosyl transferase family 20 suggested the existence of Citrus paralogs. Analysis of the Citrus gene space showed that the most important metabolic pathways known to affect fruit quality were represented in the unigene set. Overall, the similarity analyses indicated that the sequences of the genes belonging to these varieties and rootstocks were essentially identical, suggesting that the differential behaviour of these species cannot be attributed to major sequence divergences. This Citrus EST assembly contributes both crucial information to discover genes of agronomical interest and tools for genetic and genomic analyses, such as the development of new markers and microarrays.</style></abstract><notes><style face="normal" font="default" size="100%">Terol, Javier Conesa, Ana Colmenero, Jose M Cercos, Manuel Tadeo, Francisco Agusti, Javier Alos, Enriqueta Andres, Fernando Soler, Guillermo Brumos, Javier Iglesias, Domingo J Gotz, Stefan Legaz, Francisco Argout, Xavier Courtois, Brigitte Ollitrault, Patrick Dossat, Carole Wincker, Patrick Morillon, Raphael Talon, Manuel Comparative Study Research Support, Non-U.S. Gov’t England BMC genomics BMC Genomics. 2007 Jan 25;8:31.</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">J. Forment</style></author><author><style face="normal" font="default" size="100%">J. Gadea</style></author><author><style face="normal" font="default" size="100%">Huerta, L.</style></author><author><style face="normal" font="default" size="100%">Abizanda, L.</style></author><author><style face="normal" font="default" size="100%">Agusti, J.</style></author><author><style face="normal" font="default" size="100%">Alamar, S.</style></author><author><style face="normal" font="default" size="100%">Alos, E.</style></author><author><style face="normal" font="default" size="100%">Andres, F.</style></author><author><style face="normal" font="default" size="100%">Arribas, R.</style></author><author><style face="normal" font="default" size="100%">Beltran, J. P.</style></author><author><style face="normal" font="default" size="100%">Berbel, A.</style></author><author><style face="normal" font="default" size="100%">Blazquez, M. A.</style></author><author><style face="normal" font="default" size="100%">Brumos, J.</style></author><author><style face="normal" font="default" size="100%">Canas, L. A.</style></author><author><style face="normal" font="default" size="100%">Cercos, M.</style></author><author><style face="normal" font="default" size="100%">Colmenero-Flores, J. M.</style></author><author><style face="normal" font="default" size="100%">A. Conesa</style></author><author><style face="normal" font="default" size="100%">Estables, B.</style></author><author><style face="normal" font="default" size="100%">Gandia, M.</style></author><author><style face="normal" font="default" size="100%">Garcia-Martinez, J. L.</style></author><author><style face="normal" font="default" size="100%">Gimeno, J.</style></author><author><style face="normal" font="default" size="100%">Gisbert, A.</style></author><author><style face="normal" font="default" size="100%">Gomez, G.</style></author><author><style face="normal" font="default" size="100%">Gonzalez-Candelas, L.</style></author><author><style face="normal" font="default" size="100%">Granell, A.</style></author><author><style face="normal" font="default" size="100%">Guerri, J.</style></author><author><style face="normal" font="default" size="100%">Lafuente, M. T.</style></author><author><style face="normal" font="default" size="100%">Madueno, F.</style></author><author><style face="normal" font="default" size="100%">Marcos, J. F.</style></author><author><style face="normal" font="default" size="100%">Marques, M. C.</style></author><author><style face="normal" font="default" size="100%">Martinez, F.</style></author><author><style face="normal" font="default" size="100%">Martinez-Godoy, M. A.</style></author><author><style face="normal" font="default" size="100%">Miralles, S.</style></author><author><style face="normal" font="default" size="100%">Moreno, P.</style></author><author><style face="normal" font="default" size="100%">Navarro, L.</style></author><author><style face="normal" font="default" size="100%">Pallas, V.</style></author><author><style face="normal" font="default" size="100%">Perez-Amador, M. A.</style></author><author><style face="normal" font="default" size="100%">Perez-Valle, J.</style></author><author><style face="normal" font="default" size="100%">Pons, C.</style></author><author><style face="normal" font="default" size="100%">Rodrigo, I.</style></author><author><style face="normal" font="default" size="100%">Rodriguez, P. L.</style></author><author><style face="normal" font="default" size="100%">Royo, C.</style></author><author><style face="normal" font="default" size="100%">Serrano, R.</style></author><author><style face="normal" font="default" size="100%">Soler, G.</style></author><author><style face="normal" font="default" size="100%">Tadeo, F.</style></author><author><style face="normal" font="default" size="100%">Talon, M.</style></author><author><style face="normal" font="default" size="100%">Terol, J.</style></author><author><style face="normal" font="default" size="100%">Trenor, M.</style></author><author><style face="normal" font="default" size="100%">Vaello, L.</style></author><author><style face="normal" font="default" size="100%">Vicente, O.</style></author><author><style face="normal" font="default" size="100%">Vidal, Ch</style></author><author><style face="normal" font="default" size="100%">Zacarias, L.</style></author><author><style face="normal" font="default" size="100%">Conejero, V.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Mol Biol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Citrus/*genetics DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Complementary/chemistry/genetics *Expressed Sequence Tags Gene Expression Profiling Gene Library *Genome</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Genomics/*methods Molecular Sequence Data Oligonucleotide Array Sequence Analysis/*methods RNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant/genetics/metabolism Reproducibility of Results Sequence Analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=15830128</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">57</style></volume><pages><style face="normal" font="default" size="100%">375-91</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis.</style></abstract><notes><style face="normal" font="default" size="100%">Forment, J Gadea, J Huerta, L Abizanda, L Agusti, J Alamar, S Alos, E Andres, F Arribas, R Beltran, J P Berbel, A Blazquez, M A Brumos, J Canas, L A Cercos, M Colmenero-Flores, J M Conesa, A Estables, B Gandia, M Garcia-Martinez, J L Gimeno, J Gisbert, A Gomez, G Gonzalez-Candelas, L Granell, A Guerri, J Lafuente, M T Madueno, F Marcos, J F Marques, M C Martinez, F Martinez-Godoy, M A Miralles, S Moreno, P Navarro, L Pallas, V Perez-Amador, M A Perez-Valle, J Pons, C Rodrigo, I Rodriguez, P L Royo, C Serrano, R Soler, G Tadeo, F Talon, M Terol, J Trenor, M Vaello, L Vicente, O Vidal, Ch Zacarias, L Conejero, V Comparative Study Research Support, U.S. Gov’t, Non-P.H.S. Netherlands Plant molecular biology Plant Mol Biol. 2005 Feb;57(3):375-91.</style></notes></record></records></xml>