%0 Journal Article %J Microb Drug Resist %D 2001 %T Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate %A Dopazo, J. %A Mendoza, A. %A Herrero, J. %A Caldara, F. %A Humbert, Y. %A Friedli, L. %A Guerrier, M. %A Grand-Schenk, E. %A Gandin, C. %A de Francesco, M. %A Polissi, A. %A Buell, G. %A Feger, G. %A Garcia, E. %A Peitsch, M. %A Garcia-Bustos, J. F. %K Bacterial Molecular Sequence Data Pneumococcal Infections/*microbiology Prokaryotic Cells RNA %K Bacterial/chemistry/genetics Genes %K Bacterial/genetics *Genome %K DNA %K Transfer/metabolism Streptococcus pneumoniae/*genetics %X The public availability of numerous microbial genomes is enabling the analysis of bacterial biology in great detail and with an unprecedented, organism-wide and taxon-wide, broad scope. Streptococcus pneumoniae is one of the most important bacterial pathogens throughout the world. We present here sequences and functional annotations for 2.1-Mbp of pneumococcal DNA, covering more than 90% of the total estimated size of the genome. The sequenced strain is a clinical isolate resistant to macrolides and tetracycline. It carries a type 19F capsular locus, but multilocus sequence typing for several conserved genetic loci suggests that the strain sequenced belongs to a pneumococcal lineage that most often expresses a serotype 15 capsular polysaccharide. A total of 2,046 putative open reading frames (ORFs) longer than 100 amino acids were identified (average of 1,009 bp per ORF), including all described two-component systems and aminoacyl tRNA synthetases. Comparisons to other complete, or nearly complete, bacterial genomes were made and are presented in a graphical form for all the predicted proteins. %B Microb Drug Resist %V 7 %P 99-125 %G eng %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11442348