%0 Book Section %B Microarray Technology Through Applications %D 2007 %T Microarray Technology in Agricultural Research %A A. Conesa %A J. Forment %A J. Gadea %A van Dijk, J. %K babelomics %B Microarray Technology Through Applications %I F. Falciani. Publisher: Taylor and Francis Group %P 173-209 %G eng %0 Journal Article %J Virology %D 2007 %T Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus %A Gandia, M. %A A. Conesa %A Ancillo, G. %A J. Gadea %A J. Forment %A Pallas, V. %A Flores, R. %A Duran-Vila, N. %A Moreno, P. %A Guerri, J. %K Citrus/*genetics/physiology/virology Closterovirus/genetics/*physiology Genes %K Genetic %K Plant Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction *Transcription %X Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P<0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress. %B Virology %V 367 %P 298-306 %G eng %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17617431 %0 Journal Article %J Plant Mol Biol %D 2005 %T Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies %A J. Forment %A J. Gadea %A Huerta, L. %A Abizanda, L. %A Agusti, J. %A Alamar, S. %A Alos, E. %A Andres, F. %A Arribas, R. %A Beltran, J. P. %A Berbel, A. %A Blazquez, M. A. %A Brumos, J. %A Canas, L. A. %A Cercos, M. %A Colmenero-Flores, J. M. %A A. Conesa %A Estables, B. %A Gandia, M. %A Garcia-Martinez, J. L. %A Gimeno, J. %A Gisbert, A. %A Gomez, G. %A Gonzalez-Candelas, L. %A Granell, A. %A Guerri, J. %A Lafuente, M. T. %A Madueno, F. %A Marcos, J. F. %A Marques, M. C. %A Martinez, F. %A Martinez-Godoy, M. A. %A Miralles, S. %A Moreno, P. %A Navarro, L. %A Pallas, V. %A Perez-Amador, M. A. %A Perez-Valle, J. %A Pons, C. %A Rodrigo, I. %A Rodriguez, P. L. %A Royo, C. %A Serrano, R. %A Soler, G. %A Tadeo, F. %A Talon, M. %A Terol, J. %A Trenor, M. %A Vaello, L. %A Vicente, O. %A Vidal, Ch %A Zacarias, L. %A Conejero, V. %K Citrus/*genetics DNA %K Complementary/chemistry/genetics *Expressed Sequence Tags Gene Expression Profiling Gene Library *Genome %K DNA %K Plant Genomics/*methods Molecular Sequence Data Oligonucleotide Array Sequence Analysis/*methods RNA %K Plant/genetics/metabolism Reproducibility of Results Sequence Analysis %X A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis. %B Plant Mol Biol %V 57 %P 375-91 %G eng %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15830128