02266nas a2200253   4500008004100000022001400041245007800055210006900133260001500202300001000217490000600227520146300233653001601696653001201712653003001724653003101754653001401785100001901799700002901818700002001847700002101867700002301888856010101911       2016                        eng d  a2045-232200aThe transcriptomics of an experimentally evolved plant-virus interaction.0 atranscriptomics of an experimentally evolved plantvirus interact  c2016 04 26  a249010 v63 a<p>Models of plant-virus interaction assume that the ability of a virus to infect a host genotype depends on the matching between virulence and resistance genes. Recently, we evolved tobacco etch potyvirus (TEV) lineages on different ecotypes of Arabidopsis thaliana, and found that some ecotypes selected for specialist viruses whereas others selected for generalists. Here we sought to evaluate the transcriptomic basis of such relationships. We have characterized the transcriptomic responses of five ecotypes infected with the ancestral and evolved viruses. Genes and functional categories differentially expressed by plants infected with local TEV isolates were identified, showing heterogeneous responses among ecotypes, although significant parallelism existed among lineages evolved in the same ecotype. Although genes involved in immune responses were altered upon infection, other functional groups were also pervasively over-represented, suggesting that plant resistance genes were not the only drivers of viral adaptation. Finally, the transcriptomic consequences of infection with the generalist and specialist lineages were compared. Whilst the generalist induced very similar perturbations in the transcriptomes of the different ecotypes, the perturbations induced by the specialist were divergent. Plant defense mechanisms were activated when the infecting virus was specialist but they were down-regulated when infecting with generalist.</p>10aArabidopsis10aEcotype10aGene Expression Profiling10aHost-Pathogen Interactions10aPotyvirus1 aHillung, Julia1 aGarcia-Garcia, Francisco1 aDopazo, Joaquin1 aCuevas, José, M1 aElena, Santiago, F  uhttp://clinbioinfosspa.es/content/transcriptomics-experimentally-evolved-plant-virus-interaction03501nas a2200469   4500008004100000022001400041245011200055210006900167260001300236300001200249490000800261520192600269653001602195653002202211653002802233653002002261653001702281653002102298653003002319653003802349653002202387653001002409653001302419653004402432653003502476653002802511653003102539653005202570653001902622653002402641653002602665653002702691100002902718700002202747700002902769700001802798700002002816700001902836700002302855700002202878856013102900       2011                        eng d  a1532-254800aEarly transcriptional defense responses in Arabidopsis cell suspension culture under high-light conditions.0 aEarly transcriptional defense responses in Arabidopsis cell susp  c2011 Jul  a1439-560 v1563 a<p>The early transcriptional defense responses and reactive oxygen species (ROS) production in Arabidopsis (Arabidopsis thaliana) cell suspension culture (ACSC), containing functional chloroplasts, were examined at high light (HL). The transcriptional analysis revealed that most of the ROS markers identified among the 449 transcripts with significant differential expression were transcripts specifically up-regulated by singlet oxygen ((1)O(2)). On the contrary, minimal correlation was established with transcripts specifically up-regulated by superoxide radical or hydrogen peroxide. The transcriptional analysis was supported by fluorescence microscopy experiments. The incubation of ACSC with the (1)O(2) sensor green reagent and 2',7'-dichlorofluorescein diacetate showed that the 30-min-HL-treated cultures emitted fluorescence that corresponded with the production of (1)O(2) but not of hydrogen peroxide. Furthermore, the in vivo photodamage of the D1 protein of photosystem II indicated that the photogeneration of (1)O(2) took place within the photosystem II reaction center. Functional enrichment analyses identified transcripts that are key components of the ROS signaling transduction pathway in plants as well as others encoding transcription factors that regulate both ROS scavenging and water deficit stress. A meta-analysis examining the transcriptional profiles of mutants and hormone treatments in Arabidopsis showed a high correlation between ACSC at HL and the fluorescent mutant family of Arabidopsis, a producer of (1)O(2) in plastids. Intriguingly, a high correlation was also observed with ABA deficient1 and more axillary growth4, two mutants with defects in the biosynthesis pathways of two key (apo)carotenoid-derived plant hormones (i.e. abscisic acid and strigolactones, respectively). ACSC has proven to be a valuable system for studying early transcriptional responses to HL stress.</p>10aArabidopsis10aBlotting, Western10aCell Culture Techniques10aCells, Cultured10aChloroplasts10aCluster Analysis10aGene Expression Profiling10aGene Expression Regulation, Plant10aHydrogen Peroxide10aLight10amutation10aOligonucleotide Array Sequence Analysis10aPhotosystem II Protein Complex10aPlant Growth Regulators10aReproducibility of Results10aReverse Transcriptase Polymerase Chain Reaction10aRNA, Messenger10aSignal Transduction10aStress, Physiological10aTranscription, Genetic1 aGonzález-Pérez, Sergio1 aGutiérrez, Jorge1 aGarcia-Garcia, Francisco1 aOsuna, Daniel1 aDopazo, Joaquin1 aLorenzo, Oscar1 aRevuelta, José, L1 aArellano, Juan, B  uhttp://clinbioinfosspa.es/content/early-transcriptional-defense-responses-arabidopsis-cell-suspension-culture-under-high-light